RUAN Jiacheng, SHl Meilin, MA Haiyan, SHl HaifengCollege of Life Sciences , Jiangsu University, Zhenjiang 212013, China)

Abstract0bjective This study aims to explore the effects of cadmium ( Cd)exposure on spleen tissuedamage and geneexpression,andexplore theunderying molecular mechanisms of cadmiumimmunotoxicity. Methods A single subeutaneous injection of CdCl,(5 wmol/kg)was used to expose themouse model. The Synergy H4 hybrid microplate reader was used to deteet malondialdehyde ( MDA )content in spleen tissue and Ca’*content in human B lymphoeytoma cells, and the differentially expressedgenes in the spleen were identified by DNA microarray analysis, GO enrichment, KEGG sinaling, andReactome pathway database were used to analyze the immune related functions of Cd exposure interference.R'T-qPCR, immunofluorescence and Western blot were used to differentially expressed genes. Results Cdexposure interferes with the autophagic function of cells , causing Ca’*homeostasis imbalance and enhaneedoxidative stress in tissues. There were 934 differentially expressed immune-related genes, including 687up-regulated genes and 247 down-regulated genes. Cd-induced four key immune genes ( Ppp3ca , Pleg2.Vavl , Cd247) regulated the innate and adaptive immune systems. Conclusion The expression changes ofkey immune regulatory genes ( Ppp3ca, Pleg2, Vavl , and Cd247) induced by cadmium exposure may bethe molecular mechanism of their immunotoxicity to mouse spleen.Keywords: cadmium ; DNA microarray; immunotoxicity ; differentially expressed genes

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