HUANG Zhiqiang, GU Shengjiu, JIN Jiamin, YANG Miao, LlU Hao, ZHU Kaimei

(College of Pharmacy, Guilin Medical University, Guilin 541199,China)

Abstract Objective To observe the elfeet of Ardisia gigantisfolia extract on the proliferation, migration, invasionand apoptosis of panereatic caneer cell line PANC-1 cells and explore its mechanism. Methods "T'he M'T'T assay wasused to test the proliferation of PANC-1 cells. The morphological change of PANC-1 cells was observed. Seratch and['ranswell migration/invasion assays were performed to test the migration and invasion abilities of PANC-l cellsWester blot analysis was conducted to examine the levels of protein expressions of p65, P-p65 , Caspase-3, Caspase9, Cleaved-Caspase-3,Cleaved-Caspase-9, Bel-2, and Bax in PANC-1 cells. Results The proliferation, migration.and invasion of PANC-1 cells were inhibited by Ardisia gigantisfolia extract, 'The protein expression levels of P-p65. Caspase-3, Caspase-9 and Bel-2 in PANC-1 cells were down-regulated by Ardisia gigantisfolia extract. The proteinexpressions of Cleaved-Caspase-3, Cleaved-Caspase-9 and Bax in PANC-l cells were up-regulated by Ardisiagigantisfolia extract. Conclusion Ardisia gigantisfolia extract inhibits the proliferation, migration and invasion ofPANC-1 cells, which the mechanism may be related to the upregulation of Bax, downregulation of Bel-2, inhibitionof p65 phosphorylation, promotion of Caspase-3 and Caspase-9 activation, and induction of apoptosis in PANC-1cells induced by Ardisia gigantisfolia extract.

Keywords : Ardisia gigantifolia extract ; pancreatic cancer; proliferation; migration and invasion; apoptosis

DOI:10.19296/j.cnki.1008-2409.2024-02-010

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